Genotyping live fish larvae: Non-lethal and noninvasive DNA isolation from 3–5 day old hatchlings

Genotyping live fish larvae: Non-lethal and noninvasive DNA isolation from 3–5 day old hatchlings

 

G. Janelle Espinoza*1,

J. Michael Poland1,

and Jaime R. Alvarado Bremer*1,2

1Department of Marine Biology, Texas A&M University, Galveston Campus, Galveston, TX
2Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX

*G.J.E. and J.R.A.B. contributed equally to this work.

BioTechniques, Vol. 63, No. 4, October 2017, pp. 181–186


Abstract

Genotyping fish larvae is a valuable technique for numerous fields of study. While methods for collecting DNA from early stage larvae have been published, a non-lethal, non-invasive genotyping protocol for hatchlings that is amenable to high-throughput approaches is desirable. Here, we describe a method to individually…

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